Wyoming led 34-31 at the break, and the game stayed close throughout the second half. The Cowboys, who led 76-75 with 1:30 remaining, sealed the win in the final seconds on two free throws and a put-back basket by Johnson.
Darnell Hugee led the Panthers (1-4) with 19 points. Derek Johnson finished with 13, and Christopher Jones and Tim Meadows scored 12 apiece.
Wyoming committed 17 turnovers, but shot better from the field—hitting 46 percent of its shots compared to 39 percent by Prairie View A&M.